J Infect Dis. 1999 Dec;180(6):2030-4. Unique Identifier : AIDSLINE
Hantavirus pulmonary syndrome (HPS) is a rare but acute fulminant
disease caused by Sin Nombre virus (SNV). To understand the role of the
viral load in the pathogenesis of HPS, the load of virus in the blood of
patients with HPS was measured. A quantitative reverse
transcription-polymerase chain reaction assay was developed for SNV,
because SNV is difficult to grow in cell culture. Thirty-eight samples
from 26 patients with HPS were analyzed. Twenty of the 26 initial
samples were positive for viral RNA (7 of 9 samples were obtained from
patients with fatal cases, and 13 of 17 were obtained from survivors).
Mean viral RNA copy numbers were 106.1+/-1.4/mL in positive cases
(106.7+/-1.4/mL in fatal cases, 105.8+/-1.3/mL in survivors) and were
correlated with peak hematocrit (P<.05) and with the lowest platelet
count (P=.05). In 8 survivors who had serial samples obtained, viral RNA
copy numbers decreased promptly after resolution of fever.
JOURNAL ARTICLE Blotting, Southern Hantavirus/*PHYSIOLOGY Hantavirus
Pulmonary Syndrome/PATHOLOGY/*VIROLOGY Hematocrit Human
Oligonucleotide Probes Plasmids/GENETICS Pulmonary Edema Reverse
Transcriptase Polymerase Chain Reaction RNA, Viral/BLOOD Support, U.S.
Gov't, P.H.S. Transcription, Genetic Viral Load Viremia/*VIROLOGY