Resource Logo
NLM AIDSLINE

Molecular analyses of the possible RNA-binding protein gene located in the human leukocyte antigen (HLA)--DR subregion.




 

Gene. 1999 Nov 15;240(1):125-32. Unique Identifier : AIDSLINE

We have previously found the sequence having potential for encoding a new protein in the human leukocyte antigen (HLA) class II region. The predicted amino acid sequence showed a significant sequence homology to the Xenopus double-stranded RNA-binding protein (Xlrbp) and the human cellular protein bound to the transactivation response (TAR) of human immunodeficiency virus type-1 (HIV-1) RNA (TRBP). Reverse transcription-polymerase chain reaction (RT-PCR) with poly(A)(+) RNA prepared from human peripheral lymphocytes and direct sequencing analyses in this study showed that the transcripts of the gene encoding the putative RNA-binding protein occurred in human cells: the gene was provisionally named Homo sapiens RNA-binding protein (hsRBP). From the result of Southern blot analyses, it appears that multiple copies of hsRBP are present in the human genome. Together with the known cDNAs having a high sequence homology to hsRBP, the data presented here suggest that the multigene family of the double-stranded RNA-binding protein exists in the human genome.

JOURNAL ARTICLE Amino Acid Sequence Base Sequence Blotting, Southern DNA/CHEMISTRY/*GENETICS Gene Dosage Gene Expression Regulation Genome, Human Histocompatibility Antigens Class II/GENETICS Human HLA-DR Antigens/*GENETICS Lymphocytes/CYTOLOGY/METABOLISM Molecular Sequence Data Multigene Family/GENETICS RNA/GENETICS RNA-Binding Proteins/*GENETICS/PHYSIOLOGY RNA, Messenger/GENETICS Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Support, Non-U.S. Gov't Transcription, Genetic Translation, Genetic



 




Information in this article was accurate in March 30, 2000. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.