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Serial analysis of gene expression in HIV-1-infected T cell lines.


FEBS Lett. 1999 Nov 26;462(1-2):182-6. Unique Identifier : AIDSLINE

The gene expression profile of the HIV-1 infection state was analyzed in the human T cell line MOLT-4. Using the serial analysis of gene expression (SAGE) method, a total of 142{ omitted}603 SAGE tags were sequenced and identified, representing 43{ omitted}581 unique mRNA species. Comparison of expression patterns revealed that 53 cellular genes were differentially expressed upon HIV-1 infection. Northern blot and RT-PCR analyses confirmed the altered expression of the genes in both MOLT-4 and MT-4 cells. Up-regulated genes were mainly composed of transcription factors and genes related to T cell activation, whereas down-regulated genes were comprised of mitochondrial proteins, actin-related factors and translational factors. These findings indicate that persistent T cell activation, which may accelerate HIV-1 replication, and the disruption of cellular housekeeping genes including those involved in anti-apoptotic systems, may play an important role in HIV-1-induced pathogenesis.

JOURNAL ARTICLE Apoptosis/GENETICS Expressed Sequence Tags *Gene Expression Profiling Human HIV Infections *HIV-1 Lymphocyte Transformation Proteins/GENETICS Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger/ANALYSIS Support, Non-U.S. Gov't T-Lymphocytes/METABOLISM/*VIROLOGY Tumor Cells, Cultured Virus Replication


Information in this article was accurate in March 30, 2000. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.