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NLM AIDSLINE

A new sulfated beta-galactan from clams with anti-HIV activity.




 

Carbohydr Res. 1999 Sep 15;321(1-2):121-7. Unique Identifier : AIDSLINE

A new polysaccharide composed of galactan sulfate with a beta-(1-->3)-glycosidic linkage has been isolated from the marine clam species Meretrix petechialis. The polysaccharide was homogeneous in its composition containing D-galactose. The glycosidic linkage was examined by 2D DQF-COSY and 2D NOESY spectroscopy. The coupling constant of anomeric proton was 7.8 Hz, suggesting a beta-galacto configuration. The downfield shift of H-2 of galactose residue demonstrated the presence of 2-O-sulfonate group. TQF-COSY confirmed that the C-6 position was substituted with a sulfonate group. The anti-HIV activity of the polysaccharides has been evaluated by the inhibition of syncytia formation. The fusion index and percentage fusion inhibition of sulfated galactan were 0.34 and 56% at 200 micrograms/mL.

JOURNAL ARTICLE Animal Anti-HIV Agents/*ISOLATION & PURIF Carbohydrate Sequence Clams/*CHEMISTRY Dextran Sulfate/PHARMACOLOGY Galactans/*ISOLATION & PURIF/PHARMACOLOGY Giant Cells/DRUG EFFECTS Hela Cells Human HIV-1/DRUG EFFECTS Membrane Fusion/DRUG EFFECTS Molecular Sequence Data Nuclear Magnetic Resonance Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.



 




Information in this article was accurate in March 30, 2000. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.