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Detection and sequencing of measles virus from peripheral mononuclear cells from patients with inflammatory bowel disease and autism.




 

Dig Dis Sci. 2000 Apr;45(4):723-9. Unique Identifier : AIDSLINE

It has been reported that measles virus may be present in the intestine of patients with Crohn's disease. Additionally, a new syndrome has been reported in children with autism who exhibited developmental regression and gastrointestinal symptoms (autistic enterocolitis), in some cases soon after MMR vaccine. It is not known whether the virus, if confirmed to be present in these patients, derives from either wild strains or vaccine strains. In order to characterize the strains that may be present, we have carried out the detection of measles genomic RNA in peripheral mononuclear cells (PBMC) in eight patients with Crohn's disease, three patients with ulcerative colitis, and nine children with autistic enterocolitis. As controls, we examined healthy children and patients with SSPE, SLE, HIV-1 (a total of eight cases). RNA was purified from PBMC by Ficoll-paque, followed by reverse transcription using AMV; cDNAs were subjected to nested PCR for detection of specific regions of the hemagglutinin (H) and fusion (F) gene regions. Positive samples were sequenced directly, in nucleotides 8393-8676 (H region) or 5325-5465 (from noncoding F to coding F region). One of eight patients with Crohn disease, one of three patients with ulcerative colitis, and three of nine children with autism, were positive. Controls were all negative. The sequences obtained from the patients with Crohn's disease shared the characteristics with wild-strain virus. The sequences obtained from the patients with ulcerative colitis and children with autism were consistent with being vaccine strains. The results were concordant with the exposure history of the patients. Persistence of measles virus was confirmed in PBMC in some patients with chronic intestinal inflammation.

JOURNAL ARTICLE Adult Autistic Disorder/*VIROLOGY Base Sequence Case-Control Studies Colitis, Ulcerative/VIROLOGY Crohn Disease/VIROLOGY DNA, Viral/*CHEMISTRY Female Human Inflammatory Bowel Diseases/*VIROLOGY Male Measles Vaccine/*ADVERSE EFFECTS Measles Virus/GENETICS/*ISOLATION & PURIF Molecular Sequence Data Monocytes/*VIROLOGY Mumps Vaccine/*ADVERSE EFFECTS Rubella Vaccine/*ADVERSE EFFECTS Vaccines, Combined/ADVERSE EFFECTS



 




Information in this article was accurate in June 30, 2000. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.