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The kinetics of specific immune responses in rhesus monkeys inoculated with live recombinant BCG expressing SIV Gag, Pol, Env, and Nef proteins.




 

Virology. 2000 Mar 1;268(1):94-103. Unique Identifier : AIDSLINE

Development of an effective preventive or therapeutic vaccine against HIV-1 is an important goal in the fight against AIDS. Effective virus clearance and inhibition of spread to target organs depends principally on the cellular immune response. Therefore, a vaccine against HIV-1 should elicit virus-specific cytotoxic lymphocyte (CTL) responses to eliminate the virus during the cell-associated stages of its life cycle. The vaccine should also be capable of inducing immunity at the mucosal surfaces, the primary route of transmission. Recombinant Bacille Calmette-Guerin (BCG) expressing viral proteins offers an excellent candidate vaccine in view of its safety and ability to persist intracellularly, resulting in the induction of long-lasting immunity and stimulation of the cellular immune response. BCG can be administered orally to induce HIV-specific immunity at the mucosal surfaces. The immunogenicity of four recombinant BCG constructs expressing simian immunodeficiency virus (SIV) Gag, Pol, Env, and Nef proteins was tested in rhesus macaques. A single simultaneous inoculation of all four recombinants elicited SIV-specific IgA and IgG antibody, and cellular immune responses, including CTL and helper T cell proliferation. Our results demonstrate that BCG recombinant vectors can induce concomitant humoral and cellular immune responses to the major proteins of SIV. Copyright 2000 Academic Press.

JOURNAL ARTICLE Animal Antibodies, Viral/*BLOOD Blotting, Western BCG Vaccine/GENETICS/IMMUNOLOGY Cloning, Molecular Cytotoxicity, Immunologic Gene Products, env/GENETICS/IMMUNOLOGY/METABOLISM Gene Products, gag/GENETICS/IMMUNOLOGY/METABOLISM Gene Products, nef/GENETICS/IMMUNOLOGY/METABOLISM Gene Products, pol/GENETICS/IMMUNOLOGY/METABOLISM IgA/BLOOD IgG/BLOOD Lymphocyte Transformation Macaca mulatta Simian Acquired Immunodeficiency Syndrome/PREVENTION & CONTROL Support, U.S. Gov't, P.H.S. SAIDS Vaccines/GENETICS/*IMMUNOLOGY SIV/GENETICS/*IMMUNOLOGY/METABOLISM T-Lymphocytes, Cytotoxic/*IMMUNOLOGY Vaccination Vaccines, Synthetic/GENETICS/*IMMUNOLOGY Viral Proteins/GENETICS/*IMMUNOLOGY/METABOLISM



 




Information in this article was accurate in June 30, 2000. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.