J Immunol. 2000 May 1;164(9):4507-12. Unique Identifier : AIDSLINE
Dendritic cells (DC) are key initiators of primary immune responses.
Myeloid DC can secrete IL-12, a potent Th1-driving factor, and are often
viewed as Th1-promoting APC. Here we show that neither a Th1- nor a
Th2-inducing function is an intrinsic attribute of human myeloid DC, but
both depend on environmental instruction. Uncommitted immature DC
require exposure to IFN-gamma, at the moment of induction of their
maturation or shortly thereafter, to develop the capacity to produce
high levels of IL-12p70 upon subsequent contact with naive Th cells.
This effect is specific for IFN-gamma and is not shared by other
IL-12-inducing factors. Type 1-polarized effector DC, matured in the
presence of IFN-gamma, induce Th1 responses, in contrast to type
2-polarized DC matured in the presence of PGE2 that induce Th2
responses. Type 1-polarized effector DC are resistant to further
modulation, which may facilitate their potential use in immunotherapy.
JOURNAL ARTICLE Cell Communication/*IMMUNOLOGY Cell
Differentiation/IMMUNOLOGY Cells, Cultured Coculture Dendritic
Cells/CYTOLOGY/*IMMUNOLOGY/*METABOLISM Dinoprostone/PHYSIOLOGY Human
Interferon Type II/BIOSYNTHESIS/PHYSIOLOGY Interleukin-12/BIOSYNTHESIS
Lymphocyte Transformation Monocytes/CYTOLOGY/*IMMUNOLOGY/METABOLISM
Support, Non-U.S. Gov't Th1 Cells/*CYTOLOGY/*IMMUNOLOGY/METABOLISM Th2