J Womens Health Gend Based Med. 2000 Apr;9(3):321-7. Unique Identifier :
The purpose of this study was to investigate whether resting energy
expenditure (REE) is elevated in early, asymptomatic human
immunodeficiency virus (HIV)-infected females and to study the
contribution of a cytokine, tumor necrosis factor-alpha (TNF-alpha), to
hypermetabolism. Cross-sectional comparison of REE in asymptomatic HIV+
females and a control group matched for age, body mass index (BMI), and
fat-free mass (FFM). Twenty-six females aged 35 +/- 7 years (10 HIV+
[mean CD4+ T cell count 636/mm3] and 16 healthy controls) participated
in this study. REE was measured by indirect calorimeter using a
Deltatrac ventilated hood with a continuous rate of 40 L/min for 30
minutes after a 40-minute equilibrium period. All tests were performed
after a 12-hour overnight fast. Twenty-four-hour urinary nitrogen was
calculated to correct for respiratory quotient. Body composition was
measured by bioelectrical impedance (BioAnalogics, Beaverton, OR).
TNF-alpha was measured by ELISA (R & D Systems, Minneapolis, MN).
Absolute REE was 17% higher (1755 kcal/kg +/- 410 versus 1497 kcal/kg
+/- 197) in the HIV+ group compared with the control group (p < 0.05).
REE remained significantly higher in the HIV+ group when REE was
adjusted for body composition differences (p = 0.04). Results revealed a
23% higher level of TNF-alpha in the HIV+ subjects (p < 0.01); however,
only a weak correlation existed between TNF-alpha and REE (r = .352).
This study documented that hypermetabolism and elevated TNF-alpha exist
in HIV+ females in the early stages of disease.
JOURNAL ARTICLE Adult Case-Control Studies Comparative Study Diet
*Energy Metabolism Female Human HIV
Infections/BLOOD/*METABOLISM/URINE Middle Age Tumor Necrosis