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Differential apoptosis effects of primate lentiviral Vpr and Vpx in mammalian cells.


J Biomed Sci. 2000 Jul-Aug;7(4):322-33. Unique Identifier : AIDSLINE

The growth inhibitory effects of Vpr and Vpx are species- and cell type-dependent. HIV-1, HIV-2 and SIV Vpr are primarily cytostatic in mammalian cells and HIV-1 Vpr has been reported to induce apoptosis in human cells. Our previous studies have shown that HIV-1, HIV-2 and SIV Vpr and Vpx have differential cytostatic and cytotoxic effects in the yeast cells [Zhang et al.: Virology, 230:103-112; 1997]. Here, we further examined the apoptosis function of HIV-1 Vpr in different species of mammalian cells and investigated if other primate lentiviral Vpr and Vpx exert similar functions. Our results show that none of the primate lentiviral Vpr or Vpx we tested induces apoptosis in nonhuman species of mammalian cells. However, HIV-1 Vpr, but not HIV-2 or SIV Vpr and/or Vpx, induced apoptosis in different types of human cell lines. Further, the apoptotic effect of HIV-1 Vpr can be distinguished from that of the human interferon-gamma, a known proapoptotic protein, that HIV-1 Vpr shows little to no paracrine and/or bystander effect. When coexpressed with Bcl-2 or Bcl-X(L), the apoptotic effect of HIV-1 Vpr became markedly attenuated. These results indicate that the apoptotic effect of HIV-1 Vpr is species-dependent and is intracellularly modulated by the Bcl-2 family of proteins. Our study also suggests that the proapoptotic function of HIV-1 Vpr is developmentally associated with human but not nonhuman primate species. Copyright 2000 National Science Council, ROC and S. Karger AG, Basel

JOURNAL ARTICLE Animal *Apoptosis/DRUG EFFECTS Cell Line Cycloheximide/PHARMACOLOGY Gene Products, vpr/GENETICS/*METABOLISM Genetic Vectors/GENETICS Human HIV-1/GENETICS HIV-2/GENETICS Interferon Type II/GENETICS/METABOLISM Kinetics *Lentiviruses, Primate/GENETICS Luminescent Proteins Microscopy, Fluorescence Multigene Family Proto-Oncogene Proteins c-bcl-2/GENETICS/METABOLISM Retroviridae Proteins/GENETICS/*METABOLISM Signal Transduction Species Specificity Support, Non-U.S. Gov't SIV/GENETICS Transfection Viral Regulatory Proteins/GENETICS/*METABOLISM


Information in this article was accurate in October 30, 2000. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.