Resource Logo
NLM AIDSLINE

Nucleotide sequence analysis identifies the human c-sis proto-oncogene as a structural gene for platelet-derived growth factor.




 

Cell. 1984 May;37(1):123-9. Unique Identifier : AIDSLINE GENBANK/K01917

The simian sarcoma virus transforming gene, v-sis, encodes a protein, p28sis , that is closely related to human platelet-derived growth factor (PDGF). The human locus related to v-sis was cloned and shown to contain at least five exons corresponding to the v-sis coding region. Nucleotide sequence analysis of these exons revealed that the predicted amino acid sequence of human c-sis differed by 6% from that of the woolly monkey-derived v-sis. These findings imply that the sis proto-oncogene has been well conserved during primate evolution. By comparison of the known amino acid sequences of PDGF peptides with the predicted human c-sis protein, it was possible to demonstrate that this human proto-oncogene is the structural gene encoding one of the two major polypeptides of this potent mitogen for connective tissue cells.

Base Sequence Cloning, Molecular Comparative Study *Genes, Structural *Genes, Viral Human Nucleic Acid Hybridization *Oncogenes Platelet-Derived Growth Factor/*GENETICS Retroviridae/*GENETICS Sarcoma Viruses, Simian/*GENETICS JOURNAL ARTICLE



 




Information in this article was accurate in September 30, 1984. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.