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Isolation of HTLV derived from Japanese adult T-cell leukemia patients in human diploid fibroblast strain IMR90 and the biological characters of the infected cells.


Int J Cancer. 1984 Jun 15;33(6):745-9. Unique Identifier : AIDSLINE

HTLV was isolated in the human diploid fibroblast strain IMR90 directly from the leukemic cells of the Japanese ATL-patients. The infection was checked by the presence of virus-specific p24 and proviral DNA. The isolation was successful in three of four patients. The patient whose virus isolation was unsuccessful contained only a defective viral genome in his leukemic cells. The infected cells produced polykaryons when co-cultured with catS + L-cells. Human leukemia HL60 cells formed clusters adherent to the infected IMR90 cells but not to uninfected IMR90 cells. The former phenomenon was blocked by the ATLL patients' serum.

Cell Nucleus/ULTRASTRUCTURE *Cell Transformation, Viral Cells, Cultured DNA, Viral/ANALYSIS Electrophoresis, Polyacrylamide Gel Female Fibroblasts/MICROBIOLOGY/ULTRASTRUCTURE Human HTLV-BLV Viruses/GENETICS/*ISOLATION & PURIF/ULTRASTRUCTURE Japan Leukemia/*MICROBIOLOGY/PATHOLOGY Male Middle Age Precipitin Tests Retroviridae Infections/*MICROBIOLOGY/PATHOLOGY Support, Non-U.S. Gov't Viral Proteins/ANALYSIS JOURNAL ARTICLE


Information in this article was accurate in October 30, 1984. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.