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Elevated adenosine deaminase and purine nucleoside phosphorylase activity in peripheral blood null lymphocytes from patients with acquired immune deficiency syndrome.




 

Blood. 1985 Jun;65(6):1318-24. Unique Identifier : AIDSLINE MED/85200286

The purine metabolic enzymes adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP) are important in lymphocyte differentiation, and genetic deficiencies of either enzyme have been associated with hereditary immunodeficiency states. Both ADA and PNP activity were measured in null cell-enriched and T cell-enriched peripheral blood lymphocytes from 16 patients with the acquired immune deficiency syndrome (AIDS), seven patients with the AIDS-related symptom complex (ARC), and seven asymptomatic homosexuals. ADA activity in nmol/10(6) lymphocytes/h was significantly elevated in null lymphocytes from AIDS (161 +/- 12) as compared with 23 healthy heterosexual controls (127 +/- 8;P less than .025). PNP activity was also significantly increased in null lymphocytes from AIDS patients (96 +/- 10;P less than .005) as well as those from ARC patients (84 +/- 11:P less than .025) relative to controls (61 +/- 5). No significant differences in enzyme activity were noted in T cell-enriched cells in any group. Along with elevated enzyme activity, AIDS patients had small yet significant increases in the percentages of HLA-DR (P less than .025), terminal deoxynucleotidyl transferase (TdT) (P less than .0001), and peanut agglutinin receptor (P less than .0001) positive lymphocytes in the null fraction compared with controls. TdT-positive cells appeared morphologically as large lymphoblasts with irregular nuclei. The data imply that the cellular immune deficiency in AIDS is not a result of deficiencies in lymphocyte ADA or PNP activity, but is more likely associated with an increase in an immature and/or activated lymphocyte subset.

Acquired Immunodeficiency Syndrome/*ENZYMOLOGY/IMMUNOLOGY Adenosine Deaminase/*METABOLISM Antigens, Surface/ANALYSIS DNA Nucleotidylexotransferase/METABOLISM Homosexuality Human Lymphocytes/CLASSIFICATION Lymphocytes, Null/*ENZYMOLOGY Male Nucleoside Deaminases/*METABOLISM Pentosyltransferases/*METABOLISM Purine-Nucleoside Phosphorylase/*METABOLISM Support, U.S. Gov't, P.H.S. T-Lymphocytes/ENZYMOLOGY JOURNAL ARTICLE



 




Information in this article was accurate in September 30, 1985. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.