Am J Clin Pathol. 1986 Aug;86(2):180-5. Unique Identifier : AIDSLINE
An enzyme immunoassay (EIA), designed to detect antibodies to human
T-cell lymphotropic virus type III (HTLV III), was evaluated. The
antibody test was found to be highly sensitive; serum from 221 of 223
(99.1%) patients with acquired immune deficiency syndrome (AIDS) was
positive for antibodies to HTLV III. In addition, the antibody test was
found to be highly specific; approximately 99.75% of 20,720 serum or
plasma samples from blood donors were negative for antibody to HTLV III.
In most cases, the Western Blot analysis agreed well with the EIA.
Eighty-one of 82 (98.8%) EIA-positive samples from patients with AIDS
were Western Blot positive. Of the EIA-positive blood donors, 21 of 36
(58%) were detected and confirmed by Western Blot analysis. A
solid-phase competitive EIA also was evaluated as an alternate
procedure. The preliminary results indicate that this immunoassay has a
high degree of sensitivity and specificity and could serve as an
alternate procedure to detect antibody to HTLV III.
Acquired Immunodeficiency Syndrome/IMMUNOLOGY Antibodies,
Viral/*ANALYSIS *Blood Donors Evaluation Studies Human HTLV-BLV
Viruses/*IMMUNOLOGY *Immunoenzyme Techniques Lymphatic
Diseases/IMMUNOLOGY JOURNAL ARTICLE