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Evaluation of HIV antibody tests in Brazil.


Int Conf AIDS. 1989 Jun 4-9;5:1012 (abstract no. W.G.P.15). Unique

OBJECTIVE: To compare the sensitivity, specificity and ease of performance of different serologic tests for HIV in panels of sera from Brazilians. METHODS: Brazilian serum panels (2 panels containing around 300 sera, half of each being HIV positive sera) were tested with the following kits: enzyme immunoassay (EIA): Abbott, Roche, Hoechst, Virgo, Organon and Salck; Virgo indirect immunofluorescence (IIF); Salck Passive Hemagglutination (PH); Embrabio dot blot (DB) and Karpas cell test (CT). The parameters evaluated were the sensitivity, specificity, time consumed, equipment necessary and serum dilutions. RESULTS: greater than or equal to 98% sensitivity: Abbott, Roche, Hoechst, Virgo EIA, Organon, Salck EIA, Virgo IIF and Embrabio DB; greater than or equal to 95% specificity: Abbott, Hoechst, Virgo EIA, Organon, Salck EIA, Virgo IIF and Embrabio DB; less than 150 minutes consumed: Abbott, Virgo EIA, Organon, Hoechst, Virgo EIA, Salck PH and Karpas CT; 2 equipment: Salck PH, Karpas CT and Embrabio CT; undiluted serum: Hoechst. CONCLUSIONS: Although several kits presented the sensitivity and specificity required by the Brazilian Ministry of Health, we think that to obtain a more accurate screening of blood it is necessary to perform lot-to-lot and proficiency evaluations. Evaluation of kits using panels constituted by endemic disease sera (Chagas' disease, schistosomiasis, leishmaniasis and malaria) are underway.

Blotting, Western Brazil Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique Hemagglutination Tests Human HIV Antibodies/*ANALYSIS Reagent Kits, Diagnostic/*STANDARDS ABSTRACT


Information in this article was accurate in September 30, 1990. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.