single point mutations in the hiv envelope gene encoding the extracellular glycoprotein affect viral tropism and receptor binding.

NLM AIDSLINE Single point mutations in the HIV envelope gene encoding the extracellular glycoprotein affect viral tropism and receptor binding. Cordonnier A; Riviere Y; Montagnier L; Emerman M; Unite d'Oncologie
September 30, 1990

Int Conf AIDS. 1989 Jun 4-9;5:526 (abstract no. W.C.O.14). Unique OBJECTIVE: To investigate the functional significance of the domains of the envelope protein that are highly conserved between HIV-1 and HIV-2. METHODS: Short deletions or point mutations were made within the highly conserved regions of the envelope gene and the mutated genes were expressed in vaccinia virus recombinants, plasmid vectors, and/or infectious HIV proviral clones. Receptor binding was analysed by co-immunoprecipitation with soluble CD4. Supernatants from transfection of proviral clones were assayed for infectivity. RESULTS: In vitro studies revealed that the integrity of at least four domains of the envelope protein was indispensable for binding to the receptor. Detailed studies of one of these regions demonstrates that a single amino acid change can be critical for binding to CD4 molecule in vitro. Mutation of another highly conserved amino acid in this region did not drastically affect receptor binding but surprisingly, the tropism of the virus particles containing this mutation was modified. CONCLUSION: Single amino acid changes in highly conserved regions of the envelope gene can have critical effects on receptor binding and cell tropism. Antigens, CD4/METABOLISM HIV Antigens/GENETICS HIV-1/GENETICS/METABOLISM HIV-2/GENETICS/METABOLISM *Mutation ABSTRACT

Most Read: Latest Added Related Topics White House LGBT Conference on HIV/AIDS UNITED STATES: White House in Atlanta: LGBT AIDS Conference No more single-theme conferences: global HIV prevention conference planned FHI 360 Senior Scientist Lut Van Damme Receives International Microbicide Conference Lifetime Achievement Award Equatorial Guinea Ministry of Health and Social Welfare and UNICEF Refute Erroneous AIDS Prevalence Figures Photographs from A Day with HIV Now Online Bulgaria urges Libya to investigate AIDS nurses case Engineers enter fight against AIDS in Africa Gilead’s Complera® Non-Inferior to Atripla® Among Treatment-Naive HIV Patients UN chief Ban calls world leaders to end Aids by 2020 OHIO: Phila Health Department HIV Testing Well-Received CONNECTICUT: City's Circle Care Center is a Model for HIV Prevention UNITED STATES: New Compound Excels at Killing Persistent and Drug-Resistant Tuberculosis INDIA: India Faces TB Drug Shortage, Warns International Medical Org ALASKA: After Topping List for Chlamydia Rates, Alaska Seeing Some Improvement Tanzania: Police Abuse, Torture, Impede HIV Services Growing HIV/AIDS awareness in Indonesia's Papua region South Africa: Catastrophe as Eastern Cape drug stock-outs continue Community-led programmes critical to progress on AIDS in Sri Lanka One in Four Surveyed EU5 Physicians Has Been Required to Change a Prescription for Some or Most of Their HIV Patients in the Past Twelve Months, Primarily to Reduce Costs OHIO: Phila Health Department HIV Testing Well-Received CONNECTICUT: City's Circle Care Center is a Model for HIV Prevention TAIWAN: HIV/AIDS Spreading to Younger People in Taiwan FLORIDA: Groups Offering Free HIV Testing INDIA: HIV Prevention Among Female Sex Workers in India Reduces HIV and Syphilis Tanzania: Police Abuse, Torture, Impede HIV Services Growing HIV/AIDS awareness in Indonesia's Papua region US program marks birth of one millionth HIV-free baby One in Four Surveyed EU5 Physicians Has Been Required to Change a Prescription for Some or Most of Their HIV Patients in the Past Twelve Months, Primarily to Reduce Costs How long can one born with HIV live?
Information in this article was accurate in September 30, 1990. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.