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Cultivation of human immunodeficiency virus from whole blood: effect of anticoagulant and inoculum size on virus growth.


J Med Virol. 1990 Jun;31(2):161-4. Unique Identifier : AIDSLINE

Human immunodeficiency virus (HIV) was cultivated directly from whole blood treated with anticoagulant by cocultivation with phytohaemagglutinin-stimulated cord blood lymphocytes. When heparin was used as the anticoagulant, isolation rates were low (10% to 56%, depending on the patient group); but when EDTA was used, isolation rates were much higher (50% to 100%). Culture of whole blood gave results identical to those of culture of separated peripheral mononuclear cells, and in some cases virus could be isolated from as little as 10 microliters of unseparated EDTA anticoagulated blood.

Adult Anticoagulants/*PHARMACOLOGY Blood/*MICROBIOLOGY Blood Specimen Collection/METHODS Comparative Study Edetic Acid/PHARMACOLOGY Fetal Blood/CYTOLOGY Heparin/PHARMACOLOGY Human HIV/GROWTH & DEVELOPMENT/*ISOLATION & PURIF Infant, Newborn Lymphocytes/*MICROBIOLOGY Support, Non-U.S. Gov't Virus Cultivation JOURNAL ARTICLE


Information in this article was accurate in November 30, 1990. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.