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Heterologous leukocyte-induced reactivation of HIV-1: implications for transfusion support of infected persons.




 

Int Conf AIDS. 1990 Jun 20-23;6(3):138 (abstract no. S.A.227). Unique

OBJECTIVE: Evaluate possible cofactor role of heterologous blood components in reactivating HIV-1 infection. METHODS: Peripheral blood mononuclear cells (PBMC) from 3 asymptomatic anti-HIV-1-positive individuals were cultured and carefully monitored for HIV-1 proviral DNA titer using end-point dilution PCR and expression (RNA,p24 Ag) under the following conditions: 1) no stimulation (negative control); 2) phytohemagglutinin (PHA) stimulation (positive control); 3) coculture with 10(1) to 10(7) PHA-stimulated or non-stimulated, mitomicin C-treated (MCT) heterologous (HLA-matched and -mismatched) PBMC: 4) coculture with heterologous plasma, platelets, and RBC. RESULTS: MCT-heterologous PBMC induced a biphasic activation of HIV-1 expression: initial upregulation of viral gene expression in in vivo infected cells, followed by in vitro dissemination of infection to previously uninfected patent cells. Induction was dose-dependent; ratios of less than 1 stimulating cell to 10(3) patient PBMC were ineffective. Virus induction was enhanced by prior PHA-activation of the MCT heterologous PBMC. Both HLA-identical and mismatched PBMC induced reactivation. Enriched populations of activated monocytes were particularly effective inducers; 10% heterologous plasma showed slight induction; platelets and RBC showed no effect. CONCLUSION: These in vitro results suggest transfused heterologous PBMC may upregulate HIV-1 expression/replication in infected recipients.

Acquired Immunodeficiency Syndrome/*PATHOLOGY Blood Platelets/DRUG EFFECTS Blood Transfusion Cells, Cultured DNA, Viral/ANALYSIS Erythrocytes/DRUG EFFECTS Gene Expression Regulation, Viral Gene Products, gag/BIOSYNTHESIS/GENETICS Human *HIV-1/DRUG EFFECTS/GENETICS Leukocytes, Mononuclear/DRUG EFFECTS/*MICROBIOLOGY Lymphocyte Transformation Mitomycins/PHARMACOLOGY Phytohemagglutinins/PHARMACOLOGY Polymerase Chain Reaction RNA, Viral/BIOSYNTHESIS Up-Regulation (Physiology) Viral Core Proteins/BIOSYNTHESIS/GENETICS *Virus Activation Virus Replication ABSTRACT



 




Information in this article was accurate in December 30, 1990. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.