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Characterisation of sensitive and resistant strains of HIV to zidovudine (AZT) in a cohort of patients at the London Hospital.


Int Conf AIDS. 1990 Jun 20-23;6(3):117 (abstract no. S.B.85). Unique

OBJECTIVES: Establish a cohort and characterise HIV isolated from serial specimens on a clinical, biological and molecular basis. METHODS: HIV isolation from plasma and lymphocytes with and without AZT. HIV quantification by supernatant P24 and syncytia. Sensitive and resistant HIV (sHIV and rHIV) characterised by: passaging in higher concentrations of AZT; back-passaging in zero AZT; fluorescent staining; neutralising assays; electron microscopy (EM); and molecular studies. RESULTS: HIV isolated from 50% of lymphocyte cultures. rHIV passaged into higher concentrations of AZT and zero AZT. Fluorescent staining underway. Neutralising and antiviral assays performed. Counts and morphology examined by EM. Molecular studies described separately. CONCLUSIONS: The cohort is established. Isolation is successful and yields resistant viruses. No correlation between isolation of rHIV from patients on or off AZT observed. No obvious biological differences between strains, other than ability to grow in the presence of AZT. Future work includes cross-resistance studies and molecular analysis.

Cells, Cultured Cohort Studies Colony Count, Microbial Drug Resistance, Microbial Great Britain Hospitals, University Human HIV/*DRUG EFFECTS/ISOLATION & PURIF/ULTRASTRUCTURE Lymphocytes/MICROBIOLOGY Microbial Sensitivity Tests Zidovudine/*THERAPEUTIC USE ABSTRACT


Information in this article was accurate in December 30, 1990. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.