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Culture and properties of Kaposi's sarcoma cells isolated from AIDS patients.




 

Int Conf AIDS. 1990 Jun 20-23;6(3):112 (abstract no. S.A.67). Unique

OBJECTIVE: To culture Kaposi sarcoma (KS) cells and determine the cell of origin as well as their growth requirements. RESULTS: KS cells, obtained either from pleural fluid or peritoneal ascites, were isolated and maintained in continuous culture for over 5 months. KS cells exhibited a characteristic pattern of reactivity to a panel of endothelial cell (EC) markers, indicating that they were endothelial-derived (positive immunoperoxidase staining for CD13, EN-4, Pal-E monoclonal antibodies and Ulex Europaeus type 1 lectin). Furthermore, these KS cells were factor VIII positive indicating vascular endothelial origin. Provision of an extracellular matrix (fibronectin, laminin or Matrigel) was essential. Compared to normal EC, a relatively low concentration of serum sustained growth of KS cells and proliferation was shown to be independent of exogenous growth factors (endothelial cell growth factor or platelet-derived growth factor). A similar profile of 35S-methionine labelled secreted proteins was observed for KS and capillary EC using SDS-PAGE and both reverse phase and gel filtration HPLC. Additionally, the pattern was distinct from that of fibroblasts. No HIV, Epstein-Barr virus (EBV), Herpes simplex virus type 1 and 2 (HSV-1, HSV-2), human papilloma virus (HPV) and cytomegalovirus (CMV) were detected in these KS cells using immunoperoxidase and polymerase chain reaction (PCR). CONCLUSION: KS cells are derived from vascular endothelium and studies using these cultures will help clarify the immunopathogenesis of this disease.

Cell Transformation, Neoplastic Endothelium, Vascular/ANALYSIS Human Polymerase Chain Reaction Sarcoma, Kaposi's/*ETIOLOGY/MICROBIOLOGY/PATHOLOGY Tumor Cells, Cultured Tumor Markers, Biological/ANALYSIS ABSTRACT



 




Information in this article was accurate in December 30, 1990. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.