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Culture and properties of Kaposi's sarcoma cells isolated from AIDS patients.


Int Conf AIDS. 1990 Jun 20-23;6(3):112 (abstract no. S.A.67). Unique

OBJECTIVE: To culture Kaposi sarcoma (KS) cells and determine the cell of origin as well as their growth requirements. RESULTS: KS cells, obtained either from pleural fluid or peritoneal ascites, were isolated and maintained in continuous culture for over 5 months. KS cells exhibited a characteristic pattern of reactivity to a panel of endothelial cell (EC) markers, indicating that they were endothelial-derived (positive immunoperoxidase staining for CD13, EN-4, Pal-E monoclonal antibodies and Ulex Europaeus type 1 lectin). Furthermore, these KS cells were factor VIII positive indicating vascular endothelial origin. Provision of an extracellular matrix (fibronectin, laminin or Matrigel) was essential. Compared to normal EC, a relatively low concentration of serum sustained growth of KS cells and proliferation was shown to be independent of exogenous growth factors (endothelial cell growth factor or platelet-derived growth factor). A similar profile of 35S-methionine labelled secreted proteins was observed for KS and capillary EC using SDS-PAGE and both reverse phase and gel filtration HPLC. Additionally, the pattern was distinct from that of fibroblasts. No HIV, Epstein-Barr virus (EBV), Herpes simplex virus type 1 and 2 (HSV-1, HSV-2), human papilloma virus (HPV) and cytomegalovirus (CMV) were detected in these KS cells using immunoperoxidase and polymerase chain reaction (PCR). CONCLUSION: KS cells are derived from vascular endothelium and studies using these cultures will help clarify the immunopathogenesis of this disease.

Cell Transformation, Neoplastic Endothelium, Vascular/ANALYSIS Human Polymerase Chain Reaction Sarcoma, Kaposi's/*ETIOLOGY/MICROBIOLOGY/PATHOLOGY Tumor Cells, Cultured Tumor Markers, Biological/ANALYSIS ABSTRACT


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