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NLM AIDSLINE

AIDS Kaposi's sarcoma-derived cells produce and respond to interleukin 6.




 

Int Conf AIDS. 1990 Jun 20-23;6(3):112 (abstract no. S.A.66). Unique

OBJECTIVE: To determine the relationship of interleukin 6 to the development of AIDS related Kaposi's sarcoma. METHODS: Cell lines derived from Kaposi's Sarcoma lesions of patients with AIDS (AIDS-KS cells) were examined for the production of IL-6 using IL-6 bioassay, immunoassay and immunoperoxidase staining. IL-6 and IL-6 receptor expression was examined by RNA slot blot hybridization. Fresh autopsy material from patients with AIDS/KS was examined for RNA production. RESULTS: The AIDS-KS cell lines (N521J and EKS 3) secreted large amounts of immunoreactive and biologically active IL-6. We found both IL-6 and IL-6 receptor (IL-6-R) RNA by slot blot hybridization analysis of these cells. The IL-6 receptor was functional, as 3H-thymidine incorporation by N521J cells increased significantly after exposure to greater than 10 units/ml of recombinant human IL-6 (rhIL-6). Antisense IL-6 oligonucleotides inhibit cell proliferation which was reversed by exogenous IL-6. We confirmed that AIDS-KS cells produced IL-6 in vivo by preparing RNA and tissue sections from involved (KS) and uninvolved skin from a patient with AIDS-KS. We detected immunoreactive IL-6 in the involved tumor areas and to a lesser extent in the surrounding normal epidermis. Slot blot hybridization showed a great excess of IL-6 and IL-6-R RNA in involved skin compared to uninvolved skin. CONCLUSIONS: These results show that both IL-6 and IL-6-R are produced by AIDS-KS cells and suggest that IL-6 may be a growth factor for AIDS-KS cells in vitro.

Acquired Immunodeficiency Syndrome/COMPLICATIONS/*GENETICS/ PATHOLOGY Blotting, Northern Human Interleukin-6/BIOSYNTHESIS/*GENETICS Receptors, Immunologic/DRUG EFFECTS/GENETICS RNA, Messenger/ANTAGONISTS & INHIB Sarcoma, Kaposi's/ETIOLOGY/*GENETICS Tumor Cells, Cultured ABSTRACT



 




Information in this article was accurate in December 30, 1990. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.