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Test system for determination of HIV-1 frameshifting efficiency in animal cells.


Biochim Biophys Acta. 1990 Aug 27;1050(1-3):288-92. Unique Identifier :

We have developed a system in animal cells which allows the quantification of frameshifting determined by specific mRNA sequences. The method is based on the expression of an N-terminally extended firefly luciferase gene which requires frameshifting in order to be translated as a functional enzyme. The systems sensitivity is such that it allows the detection of even low efficiency of frameshifting. Our results show that the HIV-1 frameshift sequence including the 3' located stem-loop structure leads to ribosomal frameshifting at a lower level than that described for in vitro systems when tested in several fibroblastoid cell lines.

beta-Galactosidase/GENETICS/METABOLISM Amino Acid Sequence Animal Base Sequence Cell Line Chromosome Deletion Codon *Frameshift Mutation Genetic Vectors Hela Cells/METABOLISM Human HIV-1/*GENETICS Luciferase/GENETICS/METABOLISM Molecular Sequence Data Restriction Mapping Ribosomes/METABOLISM RNA, Messenger/*GENETICS Support, Non-U.S. Gov't *Transfection JOURNAL ARTICLE


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