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Test system for determination of HIV-1 frameshifting efficiency in animal cells.




 

Biochim Biophys Acta. 1990 Aug 27;1050(1-3):288-92. Unique Identifier :

We have developed a system in animal cells which allows the quantification of frameshifting determined by specific mRNA sequences. The method is based on the expression of an N-terminally extended firefly luciferase gene which requires frameshifting in order to be translated as a functional enzyme. The systems sensitivity is such that it allows the detection of even low efficiency of frameshifting. Our results show that the HIV-1 frameshift sequence including the 3' located stem-loop structure leads to ribosomal frameshifting at a lower level than that described for in vitro systems when tested in several fibroblastoid cell lines.

beta-Galactosidase/GENETICS/METABOLISM Amino Acid Sequence Animal Base Sequence Cell Line Chromosome Deletion Codon *Frameshift Mutation Genetic Vectors Hela Cells/METABOLISM Human HIV-1/*GENETICS Luciferase/GENETICS/METABOLISM Molecular Sequence Data Restriction Mapping Ribosomes/METABOLISM RNA, Messenger/*GENETICS Support, Non-U.S. Gov't *Transfection JOURNAL ARTICLE



 




Information in this article was accurate in January 30, 1991. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.