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The viral envelope gene is involved in macrophage tropism of a human immunodeficiency virus type 1 strain isolated from brain tissue.


J Virol. 1990 Dec;64(12):6148-53. Unique Identifier : AIDSLINE

Human immunodeficiency virus type 1 (HIV-1) strains isolated from the central nervous system (CNS) may represent a subgroup that displays a host cell tropism different from those isolated from peripheral blood and lymph nodes. One CNS-derived isolate, HIV-1SF128A, which can be propagated efficiently in primary macrophage culture but not in any T-cell lines, was molecularly cloned and characterized. Recombinant viruses between HIV-1SF128A and the peripheral blood isolate HIV-1SF2 were generated in order to map the viral gene(s) responsible for the macrophage tropism. The env gene sequences of the two isolates are about 91.1% homologous, with variations scattered mainly in the hypervariable regions of gp120. Recombinant viruses that have acquired the HIV-1SF128A env gene display HIV-1SF128A tropism for macrophages. Furthermore, the gp120 variable domains, V1, V2, V4, and V5, the CD4-binding domain, and the gp41 fusion domain are not directly involved in determining macrophage tropism.

Acquired Immunodeficiency Syndrome/COMPLICATIONS/MICROBIOLOGY Amino Acid Sequence Brain/*MICROBIOLOGY Cells, Cultured Cloning, Molecular Comparative Study Dementia/ETIOLOGY *Genes, Structural, Viral Human HIV-1/*GENETICS/ISOLATION & PURIF Leukocytes, Mononuclear/IMMUNOLOGY Molecular Sequence Data Restriction Mapping Sequence Homology, Nucleic Acid Spinal Cord/MICROBIOLOGY Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Transfection Viral Envelope Proteins/*GENETICS JOURNAL ARTICLE


Information in this article was accurate in March 30, 1991. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.