J Infect Dis. 1991 May;163(5):1008-15. Unique Identifier : AIDSLINE
Epstein-Barr virus (EBV) obtained directly from the oropharynx was used
to detect viral DNA deleted for the EBV nuclear antigen 2
(EBNA2)-encoding gene that is essential for lymphocyte transformation.
By polymerase chain reaction analysis, the deletion was found in virus
from 5 of 33 healthy adult donors and 11 of 12 patients with concurrent
human immunodeficiency virus infection. Lymphoblastoid cell lines that
produce standard transforming EBV also harbored EBNA2-deleted virus in
cells permissive of EBV replication. In vitro infectivity studies
indicated that the DNA is packaged and transmissible, with biologic
properties similar to those of a laboratory mutant, P3HR-1, which also
lacks the EBNA2 gene. These findings, obtained from productively
infected cell systems, provide evidence for the existence in nature of a
transformation-incompetent EBV variant that may facilitate EBV
persistence and the emergence of reactivation diseases.
Antigens, Viral/*GENETICS Base Sequence Cell Line Cell
Nucleus/IMMUNOLOGY Cell Transformation, Viral Chromosome Deletion
DNA, Viral/ANALYSIS/CHEMISTRY Herpesviridae Infections/*MICROBIOLOGY
Herpesvirus 4, Human/*GENETICS/IMMUNOLOGY/PHYSIOLOGY Human Molecular
Sequence Data Mutation Nucleic Acid Hybridization
Oropharynx/MICROBIOLOGY Polymerase Chain Reaction Support, Non-U.S.
Gov't Support, U.S. Gov't, P.H.S. Virus Replication JOURNAL ARTICLE