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Identification and analysis of antisense RNA target regions of the human immunodeficiency virus type 1.




 

Nucleic Acids Res. 1991 Apr 11;19(7):1421-6. Unique Identifier :

Antisense RNA, transcribed intracellularly from constitutive expression cassettes, inhibits the replication of the human immunodeficiency virus type 1 (HIV-1) as demonstrated by a quantitative microinjection assay in human SW480 cells. Infectious proviral HIV-1 DNA was co-microinjected together with a fivefold molar excess of plasmids expressing antisense RNA complementary to a set of ten different HIV-1 target regions. The most inhibitory antisense RNA expression plasmids were targeted against a 1 kb region within the gag open reading frame and against a 562 base region containing the coding sequences for the regulatory viral proteins tat and rev. Experimental evidence is presented that the antisense principle is the inhibitory mechanism in this assay system.

Cell Line Cloning, Molecular Human HIV Antigens/ANALYSIS HIV-1/*GENETICS/PHYSIOLOGY Microinjections Plasmids RNA, Antisense/*GENETICS RNA, Viral/*GENETICS Substrate Specificity Support, Non-U.S. Gov't Virus Replication JOURNAL ARTICLE



 




Information in this article was accurate in August 30, 1991. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.