Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):363-7. Unique Identifier :
In studies on viral interference, cloned T-cell lines chronically
infected with human immunodeficiency virus (HIV) type 1 or HIV-2 were
inoculated with several strains of these two AIDS retrovirus subtypes.
HIV-2UC1-infected cells, which still express the CD4 receptor, could be
superinfected with a variety of HIV-1 and HIV-2 strains. This event was
accompanied by cytopathic effects in the cells and production of
pseudotype virions with an expanded cellular host range. HIV-1- or
HIV-2-infected clonal cell lines, which did not express CD4, could not
be superinfected by any HIV strains but were coinfected after
transfection of molecular clones into the persistently infected cells.
These observations indicate that viral interference with HIV occurs at
the cell surface and involves a down-modulation of the CD4 molecule. If
the CD4 protein is expressed, superinfection can take place, and
phenotypically mixed virus particles are produced. Since HIV-1 and HIV-2
dually infected individuals have been detected, these in vitro
observations may have relevance to the in vivo state.
B-Lymphocytes/MICROBIOLOGY Cytopathogenic Effect, Viral CD4-Positive
T-Lymphocytes/*MICROBIOLOGY Human HIV Antigens/ANALYSIS HIV
Infections/*MICROBIOLOGY HIV-1/*GROWTH & DEVELOPMENT/IMMUNOLOGY
HIV-2/*GROWTH & DEVELOPMENT/IMMUNOLOGY In Vitro Neutralization Tests
Phenotype Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S.
Transfection Virus Replication JOURNAL ARTICLE