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Site-specific hydrolysis and alcoholysis of human immunodeficiency virus DNA termini mediated by the viral integrase protein.


Nucleic Acids Res. 1991 Dec 25;19(24):6691-8. Unique Identifier :

Before integration of the human immunodeficiency virus (HIV) DNA, two nucleotides are removed from the 3' ends of the viral DNA by the integrase (IN) protein. We studied the chemistry of this reaction, and found that IN mediates site-specific hydrolysis of a phosphodiester bond, resulting in release of a dinucleotide. A class of alcohols (including glycerol, 1,2-propanediol, but not 1,3-propanediol) can also act as nucleophile in this reaction, and likewise the alcoholic amino acids L-serine and L-threonine can be covalently linked to the dinucleotide. No evidence was found for a covalent linkage between the IN protein and this dinucleotide, suggesting that IN directs a single nucleophilic attack of water at the specific phosphodiester bond.

Base Sequence DNA Nucleotidyltransferases/*METABOLISM DNA, Viral/*METABOLISM Glycerin/METABOLISM Human Hydrolysis HIV-1/ENZYMOLOGY/GENETICS/*METABOLISM HIV-2/ENZYMOLOGY/GENETICS/*METABOLISM Molecular Sequence Data Nucleotides/METABOLISM Propanediols/METABOLISM Sarcoma Viruses, Avian/METABOLISM Sensitivity and Specificity Serine/METABOLISM Support, Non-U.S. Gov't Threonine/METABOLISM Viral Proteins/*METABOLISM JOURNAL ARTICLE


Information in this article was accurate in April 30, 1992. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.