Unfallchirurgie. 1992 Feb;18(1):1-6. Unique Identifier : AIDSLINE
In our study we examined bone disinfection by ethanol and by
irradiation. A 70% aqueous ethanol solution diffused through a 3 mm and
a 6 mm slice of human cancellous bone against 2 ml of a HIV-sample (RTA:
300,000 cpm/ml) for 24 hours. In both cases HIV could not be
inactivated. Infected T-lymphocyte cultures showed specific
morphological cell changes. The Abbott HIV-antigen-EIA proved the
treated HIV-samples to be infectious after cultivation in
macrophage-cultures. Additional gas chromatography measurements of
ethanol diffusion through 3 mm and 6 mm of human cancellous bone
supported these observations: a 70% aqueous ethanol solution achieved a
concentration of 25.6% (18.0%) in median after 24 hours and a thickness
of 3 mm (6 mm). The effect of different doses of irradiation on
HIV-samples (RTA:300,000 cpm/ml) was examined. The samples were
irradiated with 2, 7, 10, 15 and 25 kGy to determine the appropriate
dose for inactivation. Irradiation with 15 kGy caused HIV inactivation
since no virus production could be detected in the macrophage culture
(Abbott HIV-antigen-EIA). The samples irradiated with 2, 7 and 10 kGy
were still infectious.
*Alcohol, Ethyl Bone and Bones/*DRUG EFFECTS/MICROBIOLOGY/RADIATION
EFFECTS Bone Transplantation Dose-Response Relationship, Radiation
English Abstract Human HIV/*DRUG EFFECTS/RADIATION EFFECTS HIV
Infections/PREVENTION & CONTROL/TRANSMISSION Immunoenzyme Techniques
Radiation Dosage Support, Non-U.S. Gov't Virus Cultivation JOURNAL
ARTICLE