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Specific NF-kappa B subunits act in concert with Tat to stimulate human immunodeficiency virus type 1 transcription.


J Virol. 1992 Jun;66(6):3883-7. Unique Identifier : AIDSLINE

NF-kappa B is a protein complex which functions in concert with the tat-I gene product to stimulate human immunodeficiency virus (HIV) transcription. To determine whether specific members of the NF-kappa B family contribute to this effect, we have examined the abilities of different NF-kappa B subunits to act with Tat-I to stimulate transcription of HIV in Jurkat T-leukemia cells. We have found that the p49(100) DNA binding subunit, together with p65, can act in concert with Tat-I to stimulate the expression of HIV-CAT plasmid. Little effect was observed with 50-kDa forms of p105 NF-kappa B or rel, in combination with p65 or full-length c-rel, which do not stimulate the HIV enhancer in these cells. These findings suggest that the combination of p49(100) and p65 NF-kappa B can act in concert with the tat-I gene product to stimulate the synthesis of HIV RNA.

Cells, Cultured Chloramphenicol Acetyltransferase/BIOSYNTHESIS/GENETICS Gene Products, tat/*METABOLISM Human HIV-1/*METABOLISM NF-kappa B/CLASSIFICATION/*METABOLISM Retroviridae Proteins, Oncogenic/METABOLISM Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. T-Lymphocytes/*METABOLISM Trans-Activation (Genetics) *Transcription, Genetic Transfection JOURNAL ARTICLE


Information in this article was accurate in August 30, 1992. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.