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A specific sequence with a bulged guanosine residue(s) in a stem-bulge-stem structure of Rev-responsive element RNA is required for trans activation by human immunodeficiency virus type 1 Rev.


J Virol. 1992 Jun;66(6):3699-706. Unique Identifier : AIDSLINE

We demonstrate that both the in vitro RNA binding and in vivo trans activation functions of human immunodeficiency virus type 1 Rev regulatory protein Rev require the presence of a 9-nucleotide 5'-CACUAUGGG-3' RNA motif on its cognate target, the Rev-responsive element RNA. For optimal Rev recognition, this sequence must be presented as a stem-bulge-stem structure and must contain at least two G's, one of which must be unpaired, and include some or all of the CACUAU sequence upstream of the three G's. Distal mutations which result in the base pairing of the G's eliminate the Rev response. The first G is crucial, but changes at the other G's are tolerated if at least one G is unpaired. The secondary structure or the three-dimensional orientation of the B1 and B2 stem-loops of the Rev-responsive element are not relevant as long as the 5'-CACUAUGGG-3' sequence is preserved, with at least one bulged G residue.

Base Sequence Consensus Sequence *Gene Expression Regulation, Viral Gene Products, rev/*METABOLISM Guanosine/METABOLISM Hela Cells Human HIV-1/*GENETICS Models, Genetic Models, Molecular Molecular Sequence Data Mutation Nucleic Acid Conformation Regulatory Sequences, Nucleic Acid/*GENETICS RNA-Binding Proteins/METABOLISM RNA, Viral/CHEMISTRY/METABOLISM Support, U.S. Gov't, P.H.S. *Trans-Activation (Genetics) JOURNAL ARTICLE


Information in this article was accurate in August 30, 1992. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.