J Virol. 1992 Jun;66(6):3531-7. Unique Identifier : AIDSLINE
We describe human immunodeficiency type 2 (HIV-2) strains which induce
cell-to-cell fusion and infect certain CD4- human cell lines. Soluble
CD4 (sCD4) induces or enhances fusion by most HIV-2 strains tested.
Soluble CD4-immunoglobulin G chimeras and conjugates of sCD4 and
antibody to the third domain of CD4 block HIV-2 fusion of CD4- cells. We
conclude that HIV-2 can enter CD4- cells via an alternative cell surface
receptor to CD4. While some strains entered efficiently, others retained
a dependency on an interaction with sCD4 to initiate changes in the
virion envelope required for membrane fusion.
Acquired Immunodeficiency Syndrome/*MICROBIOLOGY Antigens,
CD4/*METABOLISM Cell Fusion/DRUG EFFECTS/*PHYSIOLOGY Cell Line DNA
Mutational Analysis Human HIV Envelope Protein gp120/METABOLISM
HIV-2/*PATHOGENICITY Receptors, HIV/METABOLISM Recombinant Fusion
Proteins/METABOLISM Rhabdomyosarcoma/MICROBIOLOGY Support, Non-U.S.
Gov't Tumor Cells, Cultured JOURNAL ARTICLE