analysis of the amino terminal presequence of the feline immunodeficiency virus glycoprotein: effect of deletions on the intracellular transport of gp95.

NLM AIDSLINE Analysis of the amino terminal presequence of the feline immunodeficiency virus glycoprotein: effect of deletions on the intracellular transport of gp95. Stephens EB; Butfiloski EJ; Monck E; Department of Infectious Diseases,
December 30, 1992

Virology. 1992 Oct;190(2):569-78. Unique Identifier : AIDSLINE The envelope glycoprotein of feline immunodeficiency virus (FIV) consists of two noncovalently associated subunits, the surface glycoprotein (SU; gp95) and the transmembrane glycoprotein (TM; gp40). An unusual feature of the open reading frame (ORF) encoding the FIV glycoprotein is the presence of an unusually long amino terminal sequence (149 amino acids, L region or n-region of the signal sequence) preceding the predicted hydrophobic signal sequence. To examine the role of this n-region in the biosynthesis of gp95, the gene-encoding signal sequence and the surface glycoprotein (gp95) were expressed using recombinant vaccinia viruses. Glycoprotein mutants were constructed with 25, 42, 73, 102, and 147 amino acids removed from the n-region. Expression studies revealed that deletion of 25-102 amino acids did not appreciably effect the biosynthesis, intracellular transport, and release of gp95 from the cell surface. In contrast, removal of 147 of 149 amino acids resulted in the gp95 that was blocked in release from the cell. These results indicate that between 3 and 47 amino acids of the n-region are required for the proper biosynthesis, processing, and release of the FIV gp95 from infected cells. Base Sequence Cloning, Molecular Gene Products, env/BIOSYNTHESIS/GENETICS/METABOLISM Hela Cells Human Immunodeficiency Virus, Feline/GENETICS Molecular Sequence Data Mutagenesis/GENETICS Polymerase Chain Reaction Recombinant Fusion Proteins/BIOSYNTHESIS/GENETICS/METABOLISM Signal Peptides/BIOSYNTHESIS/GENETICS/METABOLISM Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Vaccinia Virus/GENETICS JOURNAL ARTICLE

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Information in this article was accurate in December 30, 1992. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.