interactions of cd4+ plasma membrane vesicles with hiv-1 and hiv-1 envelope glycoprotein-expressing cells.

NLM AIDSLINE Interactions of CD4+ plasma membrane vesicles with HIV-1 and HIV-1 envelope glycoprotein-expressing cells. Puri A; Dimitrov DS; Golding H; Blumenthal R; Section on Membrane
December 30, 1992

J Acquir Immune Defic Syndr. 1992;5(9):915-20. Unique Identifier : To study interactions between the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (gp120-gp41) and the receptor in the target membrane, CD4, a new experimental system utilizing CD4-carrying plasma membrane vesicles (CD4 PMVs) was developed. CD4 PMVs were prepared by hypotonic lysis of HeLa cells expressing CD4 after infection with recombinant vaccinia virus containing the CD4 cDNA. The CD4 PMVs carried up to 680 CD4 molecules per vesicle. Their fusion with cells expressing gp120-gp41 after infection with recombinant vaccinia virus was monitored by fluorescence video microscopy by using lipophilic fluorescent dyes. Fluorescence changes as a result of fusion occurred within 30 min at 37 degrees C, and little fluorescence changes were seen with cells expressing the noncleaved HIV-1 envelope glycoprotein (gp160). The preincubation of CD4 PMVs with HIV-1 reduced its infectivity 10-fold. The CD4 PMVs were more effective in inhibiting syncytia formation than sCD4. These results demonstrate that CD4 PMVs could be used to study the mechanisms of HIV-1 envelope-mediated fusion and have the potential to inactivate HIV-1. Animal Antigens, CD4/METABOLISM Cell Line Cell Membrane/METABOLISM Hela Cells Human HIV Envelope Protein gp120/METABOLISM HIV Envelope Protein gp41/METABOLISM HIV-1/*METABOLISM Microscopy, Fluorescence Support, U.S. Gov't, P.H.S. JOURNAL ARTICLE

Most Read: Latest Added Related Topics White House LGBT Conference on HIV/AIDS UNITED STATES: White House in Atlanta: LGBT AIDS Conference No more single-theme conferences: global HIV prevention conference planned FHI 360 Senior Scientist Lut Van Damme Receives International Microbicide Conference Lifetime Achievement Award Equatorial Guinea Ministry of Health and Social Welfare and UNICEF Refute Erroneous AIDS Prevalence Figures Photographs from A Day with HIV Now Online Bulgaria urges Libya to investigate AIDS nurses case Engineers enter fight against AIDS in Africa Gilead’s Complera® Non-Inferior to Atripla® Among Treatment-Naive HIV Patients UN chief Ban calls world leaders to end Aids by 2020 HIV messages should include MSM Stock-outs must be top of agenda TEXAS: Free HIV Testing CALIFORNIA: Entertainment AIDS Alliance (EAA) Presents 11th Annual Wine and Wisdom Fundraiser NORTH CAROLINA: ALFA Offers Free Community Health Educator Training in Hickory CALIFORNIA: Over Half of People Living with AIDS in San Francisco Are Aged 50 or Older THAILAND; UNITED STATES: HIV Drug Can Also Protect Injection Drug Users INDONESIA: Growing HIV/AIDS Awareness in Indonesia's Papua Region UNITED STATES: Many Young Americans Unaware They're Infected with HIV Board to Vote on Condoms in Boston Schools HIV messages should include MSM TEXAS: Free HIV Testing THAILAND; UNITED STATES: HIV Drug Can Also Protect Injection Drug Users INDONESIA: Growing HIV/AIDS Awareness in Indonesia's Papua Region UNITED STATES: Many Young Americans Unaware They're Infected with HIV New International Report on HIV Prevention Urges Localized Consideration of Antiretroviral Strategies New Analysis of World's Premier AIDS Conference Finds Poor Coverage of Populations Most-at-Risk for HIV GREECE: Doubled HIV Cases Add to Greek Woes OHIO: Phila Health Department HIV Testing Well-Received CONNECTICUT: City's Circle Care Center is a Model for HIV Prevention
Information in this article was accurate in December 30, 1992. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.