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NLM AIDSLINE

Application of the polymerase chain reaction to HIV diagnostics.




 

Int Conf AIDS. 1992 Jul 19-24;8(3):41 (abstract no. PuA 6183). Unique

OBJECTIVE: Optimization of the polymerase chain reaction (PCR) method for reliable detection of HIV-1 provirus in DNA isolated from peripheral blood mononuclear cells of persons seronegative and seropositive to HIV-1, persons with indeterminate results in Western blot and persons belonging to high risk groups. METHODS: The selection of most suitable outward and nested primer pairs was performed by a developed computer program according to a number of optimization criteria. RESULTS: Two-step PCR using the optimal outward and nested primer pairs that amplify 250 and 100 base pairs sequences in the GAG region of HIV-1 permitted to decrease the possible false priming and the amplification of nonspecific sequences and helped to avoid false positive results. CONCLUSION: The computer program allowed to choose the best primer pairs for nested PCR amplification of HIV-1 target sequences and thus to increase the specificity of PCR for HIV diagnostics.

Acquired Immunodeficiency Syndrome/*DIAGNOSIS/EPIDEMIOLOGY Blotting, Western/METHODS DNA, Viral/*BLOOD/GENETICS/ISOLATION & PURIF False Positive Reactions Human HIV-1/GENETICS/*ISOLATION & PURIF Polymerase Chain Reaction/*METHODS Risk Factors Software ABSTRACT



 




Information in this article was accurate in December 30, 1992. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.