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Small amino acid changes in the V3 hypervariable region of gp120 can affect the T-cell-line and macrophage tropism of human immunodeficiency virus type 1.




 

Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9434-8. Unique Identifier :

Human immunodeficiency virus type 1 (HIV-1) strains display a high degree of heterogeneity in their biological properties that correlate with in vivo pathogenesis of the virus. We previously demonstrated that overlapping regions encompassing the third hypervariable domain (V3), within the envelope glycoprotein gp120 determine the tropisms of HIV-1 for T-cell lines and primary macrophages. Studies with mutant viruses carrying one or more amino acid substitutions in the V3 loop have now identified this hypervariable domain as a major determinant for these cellular host range properties. Three to five amino acid changes in this domain, but rarely a single amino acid substitution, can confer macrophage tropism and alter T-cell-line tropism. These findings emphasize the effect on cell tropism of small amino acid differences in the viral envelope and suggest that the overall conformation of the V3 loop plays the major role in determining the ability of HIV-1 to infect T-cell lines and primary macrophages.

Amino Acid Sequence Human HIV Envelope Protein gp120/*CHEMISTRY HIV-1/GROWTH & DEVELOPMENT/*PATHOGENICITY In Vitro Macrophages/*MICROBIOLOGY Molecular Sequence Data Mutagenesis, Site-Directed Structure-Activity Relationship Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. T-Lymphocytes/*MICROBIOLOGY Virus Replication JOURNAL ARTICLE



 




Information in this article was accurate in January 30, 1993. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.