Pept Res. 1992 Nov-Dec;5(6):315-20. Unique Identifier : AIDSLINE
A fully automated instrument for multiple simultaneous peptide synthesis
was constructed to provide large numbers of peptides for immunological
research. The synthesis is performed in a flow-through mode with the
conventional solid supports contained in 48 individual reaction columns.
The instrument is based on a commercial autosampler equipped with a
motor-driven syringe for accurate delivery of reagents and a robot arm
carrying a dispenser needle. Dedicated software was developed to compile
overlapping peptides from a given protein sequence and to control all
functions of the robot. In situ activation by BOP was chosen as the
optimized chemistry protocol. The peptides are cleaved from the resin in
the reactors used for synthesis, thus minimizing handling. Performance
of the instrument was demonstrated by synthesis of overlapping 14-mer
peptides derived from the sequence of HIV reversed transcriptase. A
second mode of operation allows the synthesis to be carried out on the
surface of polyethylene pins. Peptides derived from the sequence of
human TNF were synthesized using this method and used to characterize
antibodies raised against the intact protein.
Amino Acid Sequence Animal Epitopes/ANALYSIS Human HIV/ENZYMOLOGY
Mice Molecular Sequence Data Peptide Fragments/CHEMICAL SYNTHESIS
Peptide Mapping Peptides/*CHEMICAL SYNTHESIS/IMMUNOLOGY
Robotics/INSTRUMENTATION RNA-Directed DNA Polymerase/CHEMICAL SYNTHESIS
Software Tumor Necrosis Factor/CHEMICAL SYNTHESIS/IMMUNOLOGY JOURNAL
ARTICLE