Am J Ophthalmol. 1993 Mar 15;115(3):285-92. Unique Identifier : AIDSLINE
We studied the clinicopathologic features of seven patients with
acquired immunodeficiency syndrome (AIDS) and ocular microsporidiosis.
All patients had decreased levels of CD4-positive cells (mean, 26/ml3)
and ocular symptoms; five had bilateral punctate epithelial keratopathy,
one had intermittent red eyes with conjunctivitis, and one had red eyes
only. Light and electron microscopy of corneal and conjunctival biopsy
and cytologic specimens and intact globes disclosed microsporidia
belonging to the genus Encephalitozoon. Because E. cuniculi and E.
hellem, the two species of the Encephalitozoon genus, are
morphologically identical, an immunofluorescent antibody technique was
used for species identification. In all seven patients, the agent was
identified as E. hellem. Pathologic examination of globes obtained after
autopsy disclosed E. hellem infection to be restricted to the corneal
and conjunctival epithelium. We studied methods for the routine
diagnosis of ocular microsporidiosis in patients with AIDS, including
the role of immunofluorescent antibody staining.
Adult Animal Antibodies, Protozoan/*ANALYSIS *AIDS-Related
Opportunistic Infections Conjunctival
Diseases/IMMUNOLOGY/PATHOLOGY/PARASITOLOGY CD4-Positive T-Lymphocytes
Encephalitozoonosis/IMMUNOLOGY/*PATHOLOGY Eye Infections,
Parasitic/IMMUNOLOGY/*PATHOLOGY Female Fluorescent Antibody Technique
Human Male Middle Age JOURNAL ARTICLE