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NLM AIDSLINE

Isolation of a cDNA clone encoding DNA-binding protein (TAXREB107) that binds specifically to domain C of the tax-responsive enhancer element in the long terminal repeat of human T-cell leukemia virus type I.




 

AIDS Res Hum Retroviruses. 1993 Feb;9(2):115-21. Unique Identifier :

Five cDNA clones for TAXREB proteins that bind to the tax-responsive enhancer element of human T-cell leukemia virus type I (HTLV-I) were isolated from a Jurkat cell cDNA library. The beta-galactosidase fusion proteins of three of these clones specifically recognized the domain C within the enhancer. One of the three cDNAs, encoding TAXREB107, contained an open reading frame with 288 amino acid residues. RNA blot analysis showed that the level of mRNA for TAXREB107 increased transiently in Jurkat cells on treatment with TPA. Immunoblot analysis showed that polyclonal antibody against TAXREB107 specifically recognized a 34-kD protein in Jurkat cells. TAXREB107 may participate in tax-mediated trans-activation of transcription.

Amino Acid Sequence Cloning, Molecular DNA/*GENETICS DNA-Binding Proteins/*GENETICS/METABOLISM Enhancer Elements (Genetics) Gene Expression/DRUG EFFECTS Gene Products, tax/*GENETICS/METABOLISM Human HTLV-I/*GENETICS Repetitive Sequences, Nucleic Acid RNA, Messenger/GENETICS Support, Non-U.S. Gov't Tetradecanoylphorbol Acetate/PHARMACOLOGY JOURNAL ARTICLE



 




Information in this article was accurate in July 30, 1993. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.