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Rapid detection of human cytomegalovirus viraemia from small volumes of heparinized whole blood.




 

Res Virol. 1993 Mar-Apr;144(2):169-71. Unique Identifier : AIDSLINE

Rapid detection of human cytomegalovirus (HCMV) from blood was performed in parallel using inoculation into human fibroblastic cells of both 500 microliters of whole blood and of buffy coat derived from 9.5 ml of blood. Of the 46 samples tested, 20 were positive for HCMV, 18 when the buffy coat was inoculated (90%) and 17 (85%) when whole blood was used. Fifteen samples were positive by both techniques. Four samples gave discordant results: in three cases, viraemia was detected only by buffy coat assay and in one case only by whole blood assay. These results suggest that HCMV isolation from blood should be performed by inoculating whole blood into cell cultures if only small blood volumes can be collected.

AIDS-Related Opportunistic Infections/DIAGNOSIS Blood Specimen Collection Bone Marrow Transplantation Cytomegalovirus/*ISOLATION & PURIF Cytomegalovirus Infections/*DIAGNOSIS Heart Transplantation Human Immunocompromised Host Kidney Transplantation Viremia/*DIAGNOSIS JOURNAL ARTICLE



 




Information in this article was accurate in September 30, 1993. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.