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Structure-function relationships of the HIV-1 envelope V3 loop tropism determinant: evidence for two distinct conformations.


AIDS. 1993 May;7(5):639-46. Unique Identifier : AIDSLINE MED/93305209

OBJECTIVE: The V3 loop of the HIV-1 envelope glycoprotein gp120 is an important determinant of HIV-1-specific cell tropism. Nine different purified envelope proteins were prepared in order to examine the association between the structure of the gp120 proteins and functional properties of HIV-1 virions differing in their tropism for T-cell lines and macrophages. RESULTS: Six monoclonal antibodies to the V3 loop reacted preferentially with T-cell line-tropic gp120 envelope proteins, and one monoclonal antibody reacted preferentially with macrophage-tropic gp120 envelope proteins. T-cell line-tropic gp120 envelope proteins were at least 10-fold more susceptible to V3 loop proteolytic cleavage by human thrombin, and 1000-fold more susceptible to V3 loop proteolytic cleavage by human mast cell tryptase than macrophage-tropic gp120 envelope proteins. CONCLUSIONS: These findings suggest that there are two distinct conformations for the V3 loop of T-cell line-tropic and macrophage-tropic gp120 envelope proteins.

Amino Acid Sequence Animal Antibodies, Monoclonal Base Sequence Drosophila melanogaster/GENETICS DNA, Viral/GENETICS Epitopes/CHEMISTRY/GENETICS Gene Expression Human HIV Envelope Protein gp120/CHEMISTRY/GENETICS/*IMMUNOLOGY HIV-1/GENETICS/*IMMUNOLOGY Macrophages/IMMUNOLOGY Molecular Sequence Data Peptide Fragments/CHEMISTRY/GENETICS/*IMMUNOLOGY Protein Conformation Serine Proteinases Structure-Activity Relationship Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. T-Lymphocytes/IMMUNOLOGY JOURNAL ARTICLE


Information in this article was accurate in October 30, 1993. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.