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Acute infection of Macaca nemestrina by human immunodeficiency virus type 1.


Virology. 1993 Aug;195(2):422-31. Unique Identifier : AIDSLINE

The pigtail macaque (Macaca nemestrina) has a marked sensitivity to infection by simian immunodeficiency virus and human immunodeficiency virus type 2 (HIV-2). On this basis, we previously studied this species' susceptibility to HIV-1 and demonstrated infection in six macaques inoculated with either cell-associated HIV-1 or cell-free virus alone. This report expands upon our initial in vitro and in vivo findings. Five laboratory-adapted and one primary clinical strain of HIV-1 replicated in vitro in human and M. nemestrina peripheral blood mononuclear cells (PBMCs). Replication was enhanced when CD4+ purified PBMCs were infected and inhibited when PBMC cultures were treated with zidovudine. All six macaques demonstrated HIV-1 infection of PBMCs from 2 to 8 weeks after inoculation but nearly all PBMC cultures were negative from weeks 10 to 40. Polymerase chain reaction showed HIV-1 gag DNA in the PBMCs of all infected macaques, including times when the macaques were culture negative. All macaques developed and maintained antibodies to gag and envelope HIV-1 proteins from week 4 after inoculation through the period of observation. Five macaques showed neutralizing antibody. These findings suggest that M. nemestrina can be infected by cell-free and cell-associated HIV-1. This model of acute HIV-1 infection may help in evaluating the pathogenesis of HIV-1 replication and candidate vaccines and therapies.

Acute Disease Animal Antibody Specificity Base Sequence Cells, Cultured CD4-Positive T-Lymphocytes/MICROBIOLOGY/ULTRASTRUCTURE DNA, Viral Human HIV Antibodies/IMMUNOLOGY HIV Infections/CEREBROSPINAL FLUID/IMMUNOLOGY/*MICROBIOLOGY HIV-1/IMMUNOLOGY/*PHYSIOLOGY Leukocytes, Mononuclear/MICROBIOLOGY/ULTRASTRUCTURE Macaca nemestrina Microscopy, Electron Molecular Sequence Data Neutralization Tests Polymerase Chain Reaction Support, U.S. Gov't, P.H.S. Virus Replication JOURNAL ARTICLE


Information in this article was accurate in October 30, 1993. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.