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A novel monoclonal antibody specifically reactive with human T-lymphotropic virus type-II (HTLV-II) envelope protein.


Int J Cancer. 1993 Jul 9;54(5):858-61. Unique Identifier : AIDSLINE

A novel monoclonal antibody (MAb), N5.4.4, specifically reactive with human T-lymphotropic virus type-II (HTLV-II) envelope glycoprotein (gp) has been developed through immunization with a synthetic peptide corresponding to the amino acid sequence 171-196 of the HTLV-II envelope protein (II-env 171-196). This MAb, which belonged to the IgG1 kappa subclass, reacted with the cytosmears of HTLV-II-infected cell lines (Si-IIA, CR-IIA-I and AS-IIA), but not with those of HTLV-I-infected cell lines (MT-1 and MT-2) or other HTLV-uninfected cell lines. On Western blot analysis, this MAb reacted with gp46 of HTLV-II lysates but not with HTLV-1 lysates. Moreover, flow-cytometric analysis revealed that this MAb recognized the native surface of only the HTLV-II-producing cells. Through application to immunohistochemical or serological method, this MAb may be of value in elucidating the pathogenesis of HTLV-II infection in comparison with HTLV-I.

Amino Acid Sequence Animal Antibodies, Monoclonal/*IMMUNOLOGY Antibody Specificity/*IMMUNOLOGY Blotting, Western Flow Cytometry Human HTLV-II Antigens/CHEMISTRY/*IMMUNOLOGY Immunoglobulins, kappa-Chain/IMMUNOLOGY Mice Mice, Inbred BALB C Molecular Sequence Data Viral Envelope Proteins/CHEMISTRY/*IMMUNOLOGY JOURNAL ARTICLE


Information in this article was accurate in October 30, 1993. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.