serological diagnosis of feline immunodeficiency virus infection based on synthetic peptides from env glycoproteins.

NLM AIDSLINE Serological diagnosis of feline immunodeficiency virus infection based on synthetic peptides from Env glycoproteins. Avrameas A; Strosberg AD; Moraillon A; Sonigo P; Pancino G; Institut
November 30, 1993

Res Virol. 1993 May-Jun;144(3):209-18. Unique Identifier : AIDSLINE Feline immunodeficiency virus (FIV) is a lentivirus which infects domestic cats, causing an acquired immunodeficiency syndrome AIDS). The aim of the present work was the development of an immunoassay for the diagnosis of FIV infection, using synthetic peptides from FIV envelope (Env) glycoproteins. Four peptides (8 to 11 amino acids long) corresponding to group-specific epitopes of FIV Env extracellular (SU) or transmembrane (TM) glycoproteins were synthesized. They were evaluated by enzyme-linked immunosorbent assay (ELISA) for immunoreactivity with sera from naturally or experimentally FIV-infected cats. One of these, P237, corresponds to a conserved nonapeptide of FIV TM, folded as a loop between two cysteines. ELISA performed with P237 on 171 sera from FIV-infected cats and 46 sera from specific-pathogen-free cats showed no false positive cases and 100% detection of infected cat sera. Moreover, 47 pet cat sera which were negative with a whole virus-based-ELISA were tested with the P237 ELISA: 2 out of 47 showed reactivity. FIV infection of these two cats was confirmed by radio-immunoprecipitation assay. Temporal studies performed on serial serum samples from experimentally infected cats detected antibodies to P237 three to five weeks after inoculation of virus. Thus, the P237 ELISA is a sensitive and specific immunoassay for early detection of antibodies to FIV. In addition, this synthetic nonapeptide is easier to produce and purify than virus preparations or recombinant proteins. Feline Acquired Immunodeficiency Syndrome/DIAGNOSIS Immunodeficiency Virus, Feline/IMMUNOLOGY Retroviridae Proteins, Oncogenic/IMMUNOLOGY Viral Envelope Proteins/IMMUNOLOGY

Most Read: Latest Added Related Topics White House LGBT Conference on HIV/AIDS UNITED STATES: White House in Atlanta: LGBT AIDS Conference No more single-theme conferences: global HIV prevention conference planned FHI 360 Senior Scientist Lut Van Damme Receives International Microbicide Conference Lifetime Achievement Award Equatorial Guinea Ministry of Health and Social Welfare and UNICEF Refute Erroneous AIDS Prevalence Figures Photographs from A Day with HIV Now Online Bulgaria urges Libya to investigate AIDS nurses case Engineers enter fight against AIDS in Africa Gilead’s Complera® Non-Inferior to Atripla® Among Treatment-Naive HIV Patients UN chief Ban calls world leaders to end Aids by 2020 CANADA: Health Officials in St. John's N.L., Inform 50 People of Tuberculosis Risk OHIO: Cleveland LGBT Community Center Offers Free Support Groups and HIV, STD Testing: The Frugal Patient CALIFORNIA: Local Church Sends HIV/AIDS Care Packs to Swaziland NEVADA: Clinics Offer Low-Cost Care for those Lacking Insurance CONNECTICUT: At Hartford Public High School, A Day Reserved for 'Healthy Relationships' ITALY: HIV Is No Barrier to Successful Liver Transplant for Cancer Treatment KENYA: Kenya Launches Universal HPV Vaccine Access to Youths UNITED STATES: Navajo Confront an Increase in New H.I.V. Infections Uganda: HIV Vaccine Trials to Start in July Uganda: Remembering Those Killed By HIV/AIDS (International AIDS Candlelight Memorial) UNITED STATES: Navajo Confront an Increase in New H.I.V. Infections Navajo Confront an Increase in New H.I.V. Infections PENNSYLVANIA: Scientists Weaken HIV Infection in Immune Cells Using Synthetic Agents THE NETHERLANDS: High Prevalence of Oral HPV Infection in Dutch Gay Men WHO says Cambodia can end HIV infections by 2020 UNITED STATES: CDC Urges Second Test to Catch Hepatitis C Infection 456 colonoscopy patients warned of infection risk Synthetic derivatives of THC may weaken HIV-1 infection to enhance antiviral therapies MINNESOTA: HIV Infections Up Sharply in St. Louis County MINNESOTA: HIV Infections Up Sharply in St. Louis County
Information in this article was accurate in November 30, 1993. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.