Int Conf AIDS. 1993 Jun 6-11;9(1):39 (abstract no. WS-A23-1). Unique
In an attempt to develop an experimental AIDS vaccine, we have used the
SIV/macaque model system to evaluate various vaccine preparations:
attenuated live SIV's: various genes were precisely deleted from the
infectious SIVmm251 genome (BK28 molecular clone) and the in vitro
growth kinetics of the mutants were tested in several cell types. Nef,
vpx and vif single mutants, and nef-vif, nefvpx double mutants were
chosen for in vivo experiments; -- live recombinant vaccines:
recombinant vaccinia viruses (VV) expressing gp140 env or gag, pol and
env were used as live vaccines to prime the immune system; --purified
pseudoparticles: VV(gag-pol-env) was used to produce SIV pseudoparticles
for a subunit vaccine; --purified recombinant proteins: gp140 env, p16
gag, p26 gag, vpx, and RT were produced from mammalian cells, E. coli or
insect cells and used in combination, either for the complete course of
vaccination, or after primary immunization with the live VVs. These
prototype vaccines were injected into rhesus macaques. The immune
response generated in the animals and the results from the challenge
experiments with the molecular clone will be discussed.
*AIDS Vaccines *SIV/IMMUNOLOGY *Viral Proteins/IMMUNOLOGY