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Diagnosis of toxoplasmosis in AIDS patients by dot blot hybridization analysis in blood samples.


Int Conf AIDS. 1993 Jun 6-11;9(1):382 (abstract no. PO-B10-1480). Unique

OBJECTIVES: To describe the detection of repetitive DNA sequences of Toxoplasma gondii in blood samples from AIDS patients with suspected toxoplasmic encephalitis. METHODS: Blood samples were collected from 12 individuals: Four of them with a presumptive diagnosis of toxoplasmosis. One with a confirmed diagnosis of cerebral tuberculosis (TB) and the seven left were taken as healthy controls. Cerebral spinal fluids (CSF) samples were obtained from the same patients evaluated for central nervous system disorders. DNA extracted from the buffy coat of the blood samples and from CSF samples were hybridized with ABGTg4 DNA probe as it was described previously (Angel, S. J. Clin. Microbiol., dec 1992, 30). Tests were performed by triplicates. RESULTS: Both blood and CSF samples were positive in the four patients that showed clinical, tomographic and biopsy evidences of CNS toxoplasmosis and they were negative in the patient that presented cerebral TB. Blood samples from healthy controls were all negative, confirming the specificity of the test. CONCLUSIONS: We described here a non invasive technique (ABGTg4 dot blot hybridization in blood samples) for confirming a presumptive diagnosis of toxoplasmosis in ADS patients. We are currently evaluating this technique for the early detection of disseminated toxoplasmosis in patients at risk.

*AIDS-Related Opportunistic Infections/DIAGNOSIS *DNA, Protozoan/BLOOD *Toxoplasma/ISOLATION & PURIF *Toxoplasmosis/DIAGNOSIS


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