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Blood isolation of Toxoplasma in HIV patients.


Int Conf AIDS. 1993 Jun 6-11;9(1):381 (abstract no. PO-B10-1474). Unique

AIMS: To assess the value of toxoplasmemia comparing two HIV populations: 11 patients with toxoplasma encephalitis (group A), and 60 patients with fever of unknown origin (group B). METHODS: Parasites were revealed from white blood cells by direct immunofluorescence using an anti-P30 monoclonal antibody at 0, 5, and 28 days of culture in human embryo lung fibroblasts. RESULTS AND CONCLUSIONS: Toxoplasma was isolated from blood of 4/11 group A AIDS patients (36.4%) and 6/60 HIV seropositive patients (10%) with fever but without organic involvement (OR 5.1, p = 0.04). The sensitivity of this assay was low (36.4%) and the specificity was even lower (10%). However HIV seropositive patients without an organic involvement cannot be considered as false positives of the test. These patients represent an early stage of the toxoplasma infection. Variables associated with toxoplasma isolation were a CD4 cell count below 100/mm3 and a positive toxoplasma IgG serology. The finding of parasitemia could obviate, if positive, an invasive diagnostical procedure. The efficacy of the test can be increased if performed in patients with CD4 cells below 100/mm3 and a positive toxoplasma IgG serology.

*Acquired Immunodeficiency Syndrome/COMPLICATIONS *AIDS-Related Opportunistic Infections/BLOOD *AIDS-Related Opportunistic Infections/DIAGNOSIS *Blood/PARASITOLOGY *Fever of Unknown Origin/BLOOD *HIV Seropositivity/COMPLICATIONS *Toxoplasma/ISOLATION & PURIF *Toxoplasmosis/DIAGNOSIS


Information in this article was accurate in November 30, 1993. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.