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Two cis-acting signals control ribosomal frameshift between human T-cell leukemia virus type II gag and pro genes.


J Virol. 1993 Oct;67(10):6273-7. Unique Identifier : AIDSLINE

The open reading frame of the human T-cell leukemia virus type II pro gene is arranged at a -1 position relative to the gag gene. Synthesis of the Gag-Pro fusion polyprotein is facilitated by ribosomal frameshift into the reading frame of the pro gene. Cloning of a synthetic 41-bp oligonucleotide corresponding to the gag-pro junction within a heterologous gene (nef of human immunodeficiency virus type I) and mutation analysis revealed that two cis-acting signals, an adenosine residue stretch and a dyad symmetry sequence, flanking the UAA termination codon, are required for efficient ribosomal frameshifting between gag and pro. The stability of the stem-loop structure is crucial for frameshifting.

Amino Acid Sequence Animal Base Sequence Cells, Cultured Cloning, Molecular DNA Mutational Analysis DNA, Viral/CHEMISTRY/*METABOLISM *Frameshift Mutation *Genes, gag Genes, nef *Genes, Viral Human HIV-1/GENETICS HTLV-II/*GENETICS Molecular Sequence Data Nucleic Acid Conformation *Open Reading Frames Ribosomes/*METABOLISM Support, Non-U.S. Gov't Terminator Regions (Genetics) Translation, Genetic Viral Proteins/BIOSYNTHESIS JOURNAL ARTICLE


Information in this article was accurate in December 30, 1993. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.