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Binding of human immunodeficiency virus type I (HIV-1) gp120 to galactosylceramide (GalCer): relationship to the V3 loop.


Virology. 1994 Jun;201(2):206-14. Unique Identifier : AIDSLINE

The primary receptor for the human immunodeficiency virus (HIV) is the CD4 molecule. However, a large body of evidence has demonstrated that some cells that do not express the CD4 receptor can be infected by HIV-1 and HIV-2, indicating that an alternative mechanism of infection must exist for some cell types. Recently it was reported that antibodies against the glycosphingolipid, galactosylceramide (Gal beta 1-1'Cer;GalCer), blocked infection of several CD4 negative cell lines derived from the brain and colon. The hypothesis that GalCer might be involved in the process of HIV entry into these cells was further supported by the finding that recombinant gp120 bound GalCer with high affinity in a high performance thin layer chromatography (HPTLC) binding assay. We have examined the interactions between GalCer and gp120, and found that the oligosaccharides that constitute a large proportion of the molecular mass of this glycoprotein are not involved in binding to this glycolipid. Furthermore, using a panel of monoclonal and monospecific antibodies we have determined that gp120 binds GalCer, and the related molecule 3' sulfo galactosylceramide (sulfatide), at a site that is conformationally close to the its principal neutralizing domain (V3 loop) or at the V3 loop itself.

Animal Antibodies, Monoclonal/IMMUNOLOGY Antigens, CD4/METABOLISM Binding Sites Enzyme-Linked Immunosorbent Assay Galactosylceramides/*METABOLISM Glycosylation Human HIV Envelope Protein gp120/IMMUNOLOGY/*METABOLISM HIV-1/*METABOLISM Peptide Fragments/IMMUNOLOGY/*METABOLISM Protein Binding Support, U.S. Gov't, P.H.S. Tumor Cells, Cultured Virus Replication JOURNAL ARTICLE


Information in this article was accurate in August 30, 1994. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.