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Human immunodeficiency virus integrase directs integration to sites of severe DNA distortion within the nucleosome core.




 

Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):5913-7. Unique Identifier :

We have examined the consequences of DNA distortion and specific histone-DNA contacts within the nucleosome for integration mediated by the human immunodeficiency virus (HIV)-encoded integrase enzyme. We find that sites of high-frequency integration cluster in the most severely deformed, kinked DNA regions within the nucleosome core. This may reflect either a preference for a wide major groove for association of the integrase or a requirement for target DNA distortion in the DNA strand transfer mechanism. Both the distortion and folding of the target DNA through packaging into nucleosomes may influence the selection of HIV integration sites within the chromosome.

Animal Base Sequence Chickens DNA/CHEMISTRY/GENETICS/*METABOLISM DNA Nucleotidyltransferases/ISOLATION & PURIF/*METABOLISM DNA, Viral/GENETICS/METABOLISM Histones/METABOLISM HIV/*ENZYMOLOGY/*GENETICS *HIV Long Terminal Repeat Models, Structural Molecular Sequence Data Nucleic Acid Conformation Nucleosomes/METABOLISM Oligodeoxyribonucleotides/CHEMICAL SYNTHESIS Substrate Specificity Support, Non-U.S. Gov't Support, U.S. Gov't, P.H.S. Templates *Virus Integration JOURNAL ARTICLE



 




Information in this article was accurate in September 30, 1994. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.