DNA Cell Biol. 1994 Apr;13(4):353-64. Unique Identifier : AIDSLINE
Primary RNA transcripts of the human T-cell leukemia virus type 1
(HTLV-1) are processed into mature mRNA by a complex series of splicing
events. In this paper, we report the finding of a novel doubly spliced
pX mRNA in two out of eight HTLV-1-infected cell lines and in one out of
13 peripheral blood mononuclear cells from HTLV-1-infected individuals.
The second splicing for this novel pX mRNA is different from that for
the known doubly spliced pX mRNA. A novel acceptor site in this splicing
was generated by a single point mutation (G to A) at nucleotide 7,337 of
the pX gene. This mRNA contained a complete open reading frame that
encodes an amino-terminal truncated p27rex protein with 189 amino acids.
A new 25-kD protein was detected in the cell lines expressing the novel
pX mRNA by an antibody against the carboxy-terminal peptide of p27rex
and was termed p25rex. Although the function of p25rex is not clear, we
clarified that p25rex is a cytoplasmic phosphoprotein and its function
is different from the transcriptional regulator function of p27rex. The
possibility that the mutated virus is replicable only in cells
coinfected with the wild type HTLV-1 may explain why the incidence of
the mutants observed here is low.
Amino Acid Sequence Base Sequence Cell Line DNA, Viral Gene
Products, rex/*GENETICS Gene Products, tax/GENETICS Human
HTLV-I/*GENETICS HTLV-I Infections/MICROBIOLOGY Leukocytes,
Mononuclear/MICROBIOLOGY Molecular Sequence Data *Point Mutation *RNA
Splicing RNA, Messenger/*GENETICS RNA, Viral/*GENETICS JOURNAL