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The Rev protein of visna virus is localized to the nucleus of infected cells.




 

Virology. 1994 Jul;202(1):485-90. Unique Identifier : AIDSLINE

Visna virus is a lentivirus of sheep that is distantly related to the human lentivirus HIV-1. Like other lentiviruses, the genome of visna virus contains multiple small open reading frames that encode viral regulatory proteins. The product of one of these regulatory genes is the visna virus Rev protein, Rev-V. In this report, immunoprecipitation of visna virus-infected cells using a specific anti-Rev-V antibody, generated to a synthetic, carboxyl-terminal peptide of Rev-V, brings down a 22.5-kDa protein identical in size to the protein expressed from a functional Rev-V cDNA clone. Examination of the phosphorylation state of Rev-V indicates that it, unlike the Rev proteins of HIV-1 and CAEV, is not efficiently phosphorylated in infected cells. Cell fractionation and immunofluorescence analysis indicate that, in contrast to a previous report, Rev-V is strongly localized to the nucleus and concentrated in nucleoli of visna virus-infected cells. In addition, Rev-V localizes similarly in several different primary cells, in particular macrophages, infected with visna virus. These data indicate that the Rev-V protein is produced during visna virus infection and is localized to the nucleolus of the infected cell.

Amino Acid Sequence Animal Cell Nucleus/*MICROBIOLOGY Cells, Cultured Fluorescent Antibody Technique Gene Products, rev/*ANALYSIS Human Molecular Sequence Data Phosphorylation Precipitin Tests Sheep Support, U.S. Gov't, P.H.S. Visna-Maedi Virus/CHEMISTRY/*ISOLATION & PURIF JOURNAL ARTICLE



 




Information in this article was accurate in September 30, 1994. The state of the art may have changed since the publication date. This material is designed to support, not replace, the relationship that exists between you and your doctor. Always discuss treatment options with a doctor who specializes in treating HIV.